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rna quick purification kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co rna quick purification kit
    Rna Quick Purification Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 3527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+quick+purification+kit/FastPure+Cell+Tissue+Total+RNA+Isolation+Kit+V2/pm41529475-147-9-13
    Average 99 stars, based on 3527 article reviews
    rna quick purification kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Shaping a pro-carcinogenic hepatic microenvironment by TCDD: An integrated approach combining network toxicology, machine learning, molecular docking, molecular dynamics and experimental validation.
    Article Snippet: .. Total RNA was extracted from liver tissues using the RNA-Quick Purification Kit (RC112–01, Vazyme, Nanjing, China) according to the manufacturer’s instructions. .. RNA concentration and purity were determined by an ultra-micro spectrophotometer (Nanodrop One, Thermo Fisher Scientific, USA).

    Article Title: Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer
    Article Snippet: The following secondary antibodies were used: HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (1:5000; SA00001-2; Proteintech; RRID: AB_2722564) and HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (1:5000; SA00001-1; Proteintech; RRID: AB_2722565). .. Total RNA was extracted using an RNA-Quick Purification Kit, and cDNA was synthesized using HiScript® II QRT SuperMix for qPCR (R222-01; Vazyme). ..

    Article Title: Identification of a 5-Gene Cuproptosis Signature Predicting the Prognosis for Colon Adenocarcinoma Based on WGCNA.
    Article Snippet: .. After being treated with DLAT overexpression plasmid for 48 h, total RNA was extracted from the HT29 cell line using the RNA Quick Purification kit (Cat# RC112-01) according to the manufacturer’s instruction purchased from Vazyme, and 500 ng of RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Cat# Q222-01) purchased from Vazyme according to the manufacturer’s instructions. .. Gene expression levels were measured by reverse transcription-quantitative polymerase chain reaction using TBGreen Premix Ex Taq (Cat# RR820Q) purchased from TaKaRa on a CFX96 Real-Time System (Bio-Rad).

    Article Title: Heat Shock Protein 90 Interactome-Mediated Proteolysis Targeting Chimera (HIM-PROTAC) Degrading Glutathione Peroxidase 4 to Trigger Ferroptosis.
    Article Snippet: Targeted protein degradation (TPD) is an emerging therapeutic paradigm aimed at eliminating the disease-causing protein with aberrant expression.. Herein, we report a new approach to inducing intracellular glutathione peroxidase 4 (GPX4) protein degradation to trigger ferroptosis by bridging the target protein to heat shock protein 90 (HSP90), termed HSP90 interactome-mediated proteolysis targeting chimera (HIM-PROTAC).. Different series of HIM-PROTACs were synthesized and evaluated, and two of them, GDCNF-2/GDCNF-11 potently induced ferroptosis via HSP90-mediated ubiquitin-proteasomal degradation of GPX4 in HT-1080 cells with DC50 values of 0.18 and 0.08 μM, respectively.

    Article Title: HSP70 Interactome-Mediated Proteolysis Targeting Chimera (HSP70-PROTAC) for Ferroptosis-Driven Cancer Treatment.
    Article Snippet: .. Subsequently, the cells were exposed to different concentrations of the test compound GDAz-3 for 24 h. Total RNA from the various cell samples was extracted using an RNA-Quick Purification Kit (#RC112, Vazyme, Nanjing, China) according to the manufacturer’s instructions. .. The concentrations of the isolated RNA samples were determined using NanoDrop.

    Article Title: UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1.
    Article Snippet: Gastric cancer peritoneal metastasis (GCPM) is an aggressive condition with poor survival, underscoring the need for new therapeutic targets.. This study investigates the role of ubiquitin-conjugating enzyme E2 D2 (UBE2D2) in gastric cancer (GC).. Analysis of clinical samples revealed that UBE2D2 is overexpressed in GC tissues and correlates with poor prognosis.

    Article Title: HSP70 Interactome‐Mediated Proteolysis Targeting Chimera (HSP70‐PROTAC) for Ferroptosis‐Driven Cancer Treatment
    Article Snippet: .. Subsequently, the cells were exposed to different concentrations of the test compound GDAz‐3 for 24 h. Total RNA from the various cell samples was extracted using an RNA‐Quick Purification Kit (#RC112, Vazyme, Nanjing, China) according to the manufacturer's instructions. .. The concentrations of the isolated RNA samples were determined using NanoDrop.

    Article Title: IGF2BP2-dependent activation of ERBB2 signaling contributes to acquired resistance to tyrosine kinase inhibitor in differentiation therapy of radioiodine-refractory papillary thyroid cancer.
    Article Snippet: Acquired drug resistance represents a major obstacle to tyrosine kinase inhibitor (TKI)-induced differentiation therapy of radioiodine-refractory papillary thyroid cancer (RR-PTC); thus, there is an urgent need to elucidate the underlying mechanisms.. Here, selumetinib-resistant PTC (PTCSR) cell lines, which were characterized by loss of sodium/iodide symporter expression, enhanced insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), and activated V-Erb-B2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2) signaling, were initially established using a dose escalation method.. Upon knockdown of IGF2BP2 in PTCSR cells, ERBB2 signaling was inhibited, and the acquired drug resistance was partially reversed.

    Synthesized:

    Article Title: Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer
    Article Snippet: The following secondary antibodies were used: HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (1:5000; SA00001-2; Proteintech; RRID: AB_2722564) and HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (1:5000; SA00001-1; Proteintech; RRID: AB_2722565). .. Total RNA was extracted using an RNA-Quick Purification Kit, and cDNA was synthesized using HiScript® II QRT SuperMix for qPCR (R222-01; Vazyme). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer
    Article Snippet: The following secondary antibodies were used: HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (1:5000; SA00001-2; Proteintech; RRID: AB_2722564) and HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (1:5000; SA00001-1; Proteintech; RRID: AB_2722565). .. Total RNA was extracted using an RNA-Quick Purification Kit, and cDNA was synthesized using HiScript® II QRT SuperMix for qPCR (R222-01; Vazyme). ..

    Article Title: UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1.
    Article Snippet: Gastric cancer peritoneal metastasis (GCPM) is an aggressive condition with poor survival, underscoring the need for new therapeutic targets.. This study investigates the role of ubiquitin-conjugating enzyme E2 D2 (UBE2D2) in gastric cancer (GC).. Analysis of clinical samples revealed that UBE2D2 is overexpressed in GC tissues and correlates with poor prognosis.

    Over Expression:

    Article Title: Identification of a 5-Gene Cuproptosis Signature Predicting the Prognosis for Colon Adenocarcinoma Based on WGCNA.
    Article Snippet: .. After being treated with DLAT overexpression plasmid for 48 h, total RNA was extracted from the HT29 cell line using the RNA Quick Purification kit (Cat# RC112-01) according to the manufacturer’s instruction purchased from Vazyme, and 500 ng of RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Cat# Q222-01) purchased from Vazyme according to the manufacturer’s instructions. .. Gene expression levels were measured by reverse transcription-quantitative polymerase chain reaction using TBGreen Premix Ex Taq (Cat# RR820Q) purchased from TaKaRa on a CFX96 Real-Time System (Bio-Rad).

    Plasmid Preparation:

    Article Title: Identification of a 5-Gene Cuproptosis Signature Predicting the Prognosis for Colon Adenocarcinoma Based on WGCNA.
    Article Snippet: .. After being treated with DLAT overexpression plasmid for 48 h, total RNA was extracted from the HT29 cell line using the RNA Quick Purification kit (Cat# RC112-01) according to the manufacturer’s instruction purchased from Vazyme, and 500 ng of RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Cat# Q222-01) purchased from Vazyme according to the manufacturer’s instructions. .. Gene expression levels were measured by reverse transcription-quantitative polymerase chain reaction using TBGreen Premix Ex Taq (Cat# RR820Q) purchased from TaKaRa on a CFX96 Real-Time System (Bio-Rad).

    Reverse Transcription:

    Article Title: Identification of a 5-Gene Cuproptosis Signature Predicting the Prognosis for Colon Adenocarcinoma Based on WGCNA.
    Article Snippet: .. After being treated with DLAT overexpression plasmid for 48 h, total RNA was extracted from the HT29 cell line using the RNA Quick Purification kit (Cat# RC112-01) according to the manufacturer’s instruction purchased from Vazyme, and 500 ng of RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Cat# Q222-01) purchased from Vazyme according to the manufacturer’s instructions. .. Gene expression levels were measured by reverse transcription-quantitative polymerase chain reaction using TBGreen Premix Ex Taq (Cat# RR820Q) purchased from TaKaRa on a CFX96 Real-Time System (Bio-Rad).

    Article Title: UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1.
    Article Snippet: Gastric cancer peritoneal metastasis (GCPM) is an aggressive condition with poor survival, underscoring the need for new therapeutic targets.. This study investigates the role of ubiquitin-conjugating enzyme E2 D2 (UBE2D2) in gastric cancer (GC).. Analysis of clinical samples revealed that UBE2D2 is overexpressed in GC tissues and correlates with poor prognosis.

    SYBR Green Assay:

    Article Title: UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1.
    Article Snippet: Gastric cancer peritoneal metastasis (GCPM) is an aggressive condition with poor survival, underscoring the need for new therapeutic targets.. This study investigates the role of ubiquitin-conjugating enzyme E2 D2 (UBE2D2) in gastric cancer (GC).. Analysis of clinical samples revealed that UBE2D2 is overexpressed in GC tissues and correlates with poor prognosis.

    Polymerase Chain Reaction:

    Article Title: UBE2D2 promotes gastric cancer progression by inhibiting ferroptosis through autophagy-dependent stabilization of CST1.
    Article Snippet: Gastric cancer peritoneal metastasis (GCPM) is an aggressive condition with poor survival, underscoring the need for new therapeutic targets.. This study investigates the role of ubiquitin-conjugating enzyme E2 D2 (UBE2D2) in gastric cancer (GC).. Analysis of clinical samples revealed that UBE2D2 is overexpressed in GC tissues and correlates with poor prognosis.

    Methylation:

    Article Title: IGF2BP2-dependent activation of ERBB2 signaling contributes to acquired resistance to tyrosine kinase inhibitor in differentiation therapy of radioiodine-refractory papillary thyroid cancer.
    Article Snippet: Acquired drug resistance represents a major obstacle to tyrosine kinase inhibitor (TKI)-induced differentiation therapy of radioiodine-refractory papillary thyroid cancer (RR-PTC); thus, there is an urgent need to elucidate the underlying mechanisms.. Here, selumetinib-resistant PTC (PTCSR) cell lines, which were characterized by loss of sodium/iodide symporter expression, enhanced insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), and activated V-Erb-B2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2) signaling, were initially established using a dose escalation method.. Upon knockdown of IGF2BP2 in PTCSR cells, ERBB2 signaling was inhibited, and the acquired drug resistance was partially reversed.



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